This study was aimed at determining the chemical and quantitative composition of flavonoids in the stem of purple coneflower (Echinacea purpurea L.) using High-Performance Liquid Chromatography (HPLC; LC-40 Nexera Lite, Shimadzu). Quercetin, rutin, gallic acid, apigenin, kaempferol, and salicylic acid standards were used for analysis, while extraction was carried out in a 96% ethanol medium using an ultrasonic bath. The results showed that salicylic acid (464.528 mg/100 g) and kaempferol (54.033 mg/100 g) were present in the highest amounts in the plant material. These polyphenols are the main metabolites responsible for the antioxidant and anti-inflammatory activity of the plant, confirming their high pharmacological and technological significance.

Purple coneflower, flavonoids, polyphenols, kaempferol

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