Oxidative stress represents a critical imbalance between the production of reactive oxygen species (ROS) and the antioxidant defense systems in living organisms. Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) play a vital role in neutralizing oxidative damage. This study explores the biochemical mechanisms, experimental evaluation, and therapeutic implications of antioxidant enzymes in mitigating oxidative stress in human and animal systems. Using spectrophotometric assays, enzyme kinetics, and comparative analysis, the research highlights the potential of natural antioxidants to enhance enzyme efficiency. The results demonstrate a significant correlation between elevated enzyme activity and reduced oxidative biomarkers, suggesting the crucial role of enzymatic regulation in maintaining cellular homeostasis.

Antioxidant Enzymes, Oxidative Stress, Superoxide Dismutase

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